Recombinant 2019-nCoV NSP1 is produced by our E.coli expression system and the target gene encoding Met1-Gly180 is expressed with a 6His tag at the C-terminus.Background:The Severe Acute Respiratory Syndrome (SARS) Coronavirus (CoV) is an enveloped, positive-stranded RNA viruses that can cause a severe respiratory disease. Its genome consists of a ∼30 kb linear, non-segmented, capped, polycistronic, polyadenylated RNA molecule, the first two-third of which is directly translated into two large polyproteins. These two polypeptides are processed into 16 non-structural proteins (nsps), forming the replicase complex, which is active in the cytoplasm in close association with cellular membranes. Nsp1 was proved to be able to suppress host gene expression by promoting host mRNA degradation and was involved in cellular chemokine deregulation. This virus evades the host innate immune response in part through the expression of its non-structural protein (nsp) 1, which inhibits both host gene expression and virus- and interferon (IFN)-dependent signaling. Thus, nsp1 is a promising target for drugs, as inhibition of nsp1 would make SARS-CoV more susceptible to the host antiviral defenses.Formulation:Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.Purity:> 85 % as determined by reducing SDS-PAGE.Endotoxin:< 1.0 EU per µg as determined by the LAL method.Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C. Avoid freeze-thaw cycles.
More Details:Sequence:Met1-Gly180Fusion Tag:C-6HisAccession:YP_009725297.1Species:VirusExpression System:E. coliMol Mass:22.7 kDaAP Mol Mass:25 kDaSynonyms:SARS-CoV 2 nsp1; SARS-CoV 2 Leader proteinDatasheet
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.Manufactured by: Elabscience
Questions about this product? Ready to place an order? Email us atorders@iwai-chem.comor give us a call: (650) 486-1541 |
"iwaichem公司成立于1947年,主要在日本东京生产和销售高纯试剂,超过60年的经营历史,zui近在生命科学领域销售有大的增长,特别是在诸如分子生物学,基因工程及免疫学。
我们和国内外450家生物技术公司有合作关系,并有70人的销售团队,2500多家研究机构和制药公司客户,iwaichem在日本有1.6亿美金的销售额。"