Recombinant 2019-nCoV NSP10 is produced by our E.coli expression system and the target gene encoding Ala1-Gln139 is expressed with a 6His tag at the N-terminus.Background:Nsp10 have shown that it is a 15-kDa protein of unknown function that has been shown to interact with itself, nsp1, and nsp7. It colocalizes with N to sites of viral replication and is essential for replication. It plays a pivotal role in viral transcription by stimulating both nsp14 3"-5" exoribonuclease and nsp16 2"-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation. Nsp10 is a critical regulator of coronavirus RNA synthesis and may play an important role in polyprotein processing.Formulation:Supplied as a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4.Purity:> 80 % as determined by reducing SDS-PAGE.Endotoxin:< 1.0 EU per µg as determined by the LAL method.Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C. Avoid freeze-thaw cycles.
More Details:Sequence:Ala1-Gln139Fusion Tag:N-6HisAccession:YP_009725306.1Species:VirusExpression System:E. coliMol Mass:17.9 kDaAP Mol Mass:18 kDaSynonyms:SARS-CoV 2 nsp10; SARS-CoV 2 Growth factor-like peptide; SARS-CoV 2 GFLDatasheet
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.Manufactured by: Elabscience
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